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Some mink become subclinically infected with only minor impact on the production.
These mink may act as carriers of the disease and pose a risk for introducing new infections in the population .
180 mg tissue was homogenised in 1300 μl ATL buffer (QIAGEN, Copenhagen, Denmark) using a 5 mm stainless steel bead (QIAGEN, Copenhagen, Denmark) in a 2 ml microcentrifuge tube and shaken for 3 min.
at 30 Hz on Tissuelyzer II (QIAGEN, Copenhagen, Denmark).
NJ (Northern Jutland), MJ (Middle Jutland), SD (Southern Denmark), Zealand (SJ), HS (the regional capital and Bornholm).
* from the beginning of April to the end of March Spleens, mesenteric lymph nodes and blood-samples from euthanized AMDV-antibody positive mink were collected by Kopenhagen Fur and submitted to The National Veterinary Institute for further processing.
raccoons, weasel, ferrets, otters, skunks and badgers [9, 12].The retrospective samples constituted thirteen samples from five farms.Information on the geographical position (expressed as the postal code which cover the area around one larger town), feed producer (FP), number of antibody-positive animals at the most recent test, and whether antibody positive animals had been detected in the farm for the past three years was recorded for each sampled farm (Table 1 and Table 2).In total, 137 mink from 73 different farms were sampled and analysed.The majority of the samples were obtained during the acute outbreaks but also a number of retrospective samples were analysed dating back to 2003.
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In Denmark, a voluntary test and stamping out policy of AMDV positive mink was initiated in 1976, and supported by legislation in 1999 in order to verify the AMDV status of all Danish mink farms.